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Biohybrid Nanoarchitectures: Protein Adaptor Based Nano-Object Assembly (PABNOA)	Functional Expansion of the Cell: De Novo Organells & Cellular Modules and Nanoreactors	Biogenic Materials: Elastine like Proteins ECM mimicking matrix proteins	Sustainable Biopolymers & Scaffolds in Regenerative Medicine
Bioorthogonal Chemistry & Bioconjugation: Unnatural Amino Acids Glycolipid Library Tool Box	Modular Molecular Tecton Library for Biogenic Materials by One-Vector-Toolbox-Platform (OVTP)	Bioprocess & Enzyme engineering Peptide Generator for Biopharmaceuticals	Biomimetic Systems Membranebiochemistry for tethered bilayer lipid membranes (tBLM)

Concept and method for the efficient design and production of biogenic materials The OVTP allows for sequential-directional ligation (SDL) and cyclic-ligation (CL) of DNA-tectons Sequential-directional ligation (SDL) and Cyclic-Ligation (CL) for multimerisation and defined arrangement of DNA-tectons I. Schematic illustration of the SDL process (on the left). The DNA-tectons prepared from the identical OVTP vectors by sequential action of the restriction enzymes SacI and EarI (donor). By the sequential action of the restriction enzymes BspQI and SacI the same plasmid is used as an acceptor for compatible DNA-tectons. II. In order to generate repetitive protein multimers a CL reaction was performed by alternating reaction temperatures with alternating enzymatic activities as a "one tube reaction" (on the right). OVTP vectors with different DNA-tectons were used as donors and acceptors for the preparation and insertion of other DNA-tectons. Tectons were added serial to each cycle as an uniform fraction or parallel as a mixed fraction at each cycle to generate homogeneous or randomly configurated complex DNA-tectons.   Combinations of defined preparations of OVTP vectors in combination with fitting DNA-tectons allows for the precise composition of DNA-tectons within the OVTP vector. Resulting vectors could subsequently serve as acceptor or donor vector for further combinations on DNA-level or for protein-tecton expression. Subsequent generation of DNA-tecton and protein-tecton libraries constituted from various building blocks Huber, M. C., Schreiber, A., Wild, W., Benz, K., Schiller, S. M. "Introducing a combinatorial DNA-toolbox platform constituting defined protein-based biohybrid materials" Biomaterials 2014, 35, 8767-8779 Schiller, Stefan M., "Protein Tectons in Synthetic Biology:  The expansion of cellular functionality combining chemical biology of small organic molecules with protein tectons - unnatural amino acids, protein based biohybird materials & de novo organelles", in Synthetic Biology, ed. Giese, B., von Gleich, A., Pade, C., Wigger, H. (Springer, 2014, ISBN 978-3-319-02782-1) Schiller, S., Huber, M. C. "Protein Assembler" 2013 PCT/EP2013/060957, WO 2013/178627

                                                      

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